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Detecting Borrelia Spirochetes: A Case Study With Validation Among Autopsy Specimens

The complex etiology of neurodegenerative disease has prompted studies on multiple mechanisms including genetic predisposition, brain biochemistry, immunological responses, and microbial insult. In particular, Lyme disease is often associated with neurocognitive impairment with variable manifestations between patients. We sought to develop methods to reliably detect Borrelia burgdorferi, the spirochete bacteria responsible for Lyme disease, in autopsy…

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Development of droplet digital PCR for the detection of Babesia microti and Babesia duncani

doi:10.1016/j.exppara.2014.12.003 Abstract Babesia spp. are obligate protozoan parasites of red blood cells. Transmission to humans occurs through bites from infected ticks or blood transfusion. Infections with B. microti account for the majority of the reported cases of human babesiosis in the USA. A lower incidence is caused by the more recently described species B. duncani….

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Tick Paralysis in a Snowshoe Hare by Ixodes pacificus Ticks in British Columbia, Canada

J Veter Sci MedAugust 2014 Volume 2 Issue 2 August 29, 2014 AbstractWe provide the first reported case of tick paralysis in a wildlife animal caused by the western blacklegged tick, Ixodes pacificus Cooley & Kohls. Six I. pacificus females and one male were collected from a feral Snowshoe Hare roaming the coastal area of southwestern British Columbia, Canada….

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Blacklegged Tick Population by Migratory Birds in Ontario, Canada

Researchers have discovered a new Lyme disease endemic area in Frontenac County that was likely started by spring migratory songbirds. This remote site, near Verona, Ontario, is located a considerable distance from roads and dwellings, which indicates that songbirds introduced immature (larva, nymphs) blacklegged ticks, the primary vector of the Lyme disease bacterium, Borrelia burgdorferi….