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Lyme disease in Canada, all you'll need to know about Lyme in Canada

First detection and molecular identification of Borrelia garinii isolated from human skin in Taiwan

Li-Lian Chao, Yi-Ju Chen and Chien-Ming Shih

Journal of Medical Microbiology, published online ahead of print on 12 November 2009.

http://dx.doi.org/10.1099/jmm.0.016626-0

Abstract

Borrelia garinii, a causative agent for Lyme disease, was detected and identified from human skin for the first time in Taiwan. Lyme disease infection was confirmed by Western immunoblot tests and isolation of Borrelia spirochetes from skin biopsy specimens.

The genetic identity of this detected spirochete was determined by analyzing the gene sequence amplified by a genospecies-specific polymerase chain reaction (PCR) assay based on the 5S (rrf)-23S (rrl) intergenic spacer amplicon gene of B. burgdorferi sensu lato. Phylogenetic analysis reveals that the sequence similarity of this detected spirochete is highly homogeneous (97.7%-98.5%) within the genospecies of B. garinii and can be distinguished clearly from other genospecies of Borrelia spirochetes.

These results provide the first convincing evidence of B. garinii spirochete detected from human skin in Taiwan and also highlight the increasing demand for investigating the existence of Lyme disease infection among patients with unusual skin lesions in the Taiwan area.

http://dx.doi.org/10.1099/jmm.0.016626-0

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