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Rapid introduction of Lyme disease spirochete, Borrelia burgdorferi sensu stricto, in Ixodes scapularis (Acari: Ixodidae) established at Turkey Point Provincial Park, Ontario, Canada

John D. Scott1., Min-Kuang Lee2, Keerthi Fernando2, Danielle R. Jorgensen2, Lance A. Durden3, and Muhammad G. Morshed2,4

1 Lyme Disease Association of Ontario, 365 St. David Street South, Fergus, Ontario, Canada N1M 2L7
2 Laboratory Services, British Columbia Centre for Disease Control, 655 West 12th Ave., Vancouver, British Columbia, Canada V5Z 4R4
3 Department of Biology and Institute of Arthropodology and Parasitology, Georgia Southern University, Statesboro, GA 30460-8056, U.S.A.
4 Department of Pathology and Laboratory Medicine, University of British Columbia, Vancouver, British Columbia, Canada V5Z 4R4
Received 30 August 2007; Accepted 30 November 2007

ABSTRACT: Borrelia burgdorferi sensu stricto (s.s.) was isolated from questing adult Ixodes scapularis Say ticks collected from Turkey Point Provincial Park (TPPP), Ontario, Canada during 2005-2006. DNA from ten (67%) of 15 pools of ticks was confirmed positive for B. burgdorferi s.s. using polymerase chain reaction (PCR) by targeting the rrf (5S)-rrl (23S) intergenic spacer region and OspA genes. This significant infection rate indicates an accelerated development of B. burgdorferi s.s. in TPPP, because this pathogen was not detected five years previously during sampling of the three motile life stages of I. scapularis. Our study provides the initial report of the presence of B. burgdorferi s.s. in TPPP, which is now endemic for Lyme disease. Ultimately, people and domestic animals are at risk of contracting Lyme disease when they frequent this park.

Journal of Vector Ecology 33 (1): 64-69. 2008.
Keyword Index: Borrelia burgdorferi, Lyme disease, ticks, Ixodes scapularis, Turkey Point Provincial Park, Ontario.

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